Rabbit Vascular Endothelial Growth Factor A (VEGFA) ELISA Kit

The Assay Genie Rabbit VEGFA ELISA Kit enables quantitative measurement of vascular endothelial growth factor A in rabbit biological samples, supporting studies of angiogenesis, tumor vascularization, wound healing, and ocular neovascularization. VEGF-A is a dimeric glycoprotein that drives endothelial cell proliferation and vascular permeability, making accurate quantification essential for cardiovascular, ophthalmic, and oncology research programs that use rabbit disease models.

This sandwich immunoassay uses anti-rabbit VEGFA capture antibodies pre-coated on a microplate, with detection via biotinylated secondary antibody and streptavidin-HRP conjugation. The assay provides a detection range of 15.6 to 1000 pg/mL with sensitivity below 7.8 pg/mL, requiring a 100 uL sample volume per well. Researchers working with rabbit models of ischemia, retinopathy, or tumor xenografts can apply this kit to serum, plasma, tissue homogenates, cell lysates, and cell culture supernatants.

The 96-well microplate format includes pre-coated wells, lyophilized recombinant standards, biotinylated detection antibody, streptavidin-HRP working solution, substrate, and stop solution. Reconstituted standards and prepared samples are incubated for binding, washed, and developed for spectrophotometric measurement. Standard curve interpolation allows VEGFA concentration determination in unknown samples. Store unopened kit components refrigerated at 2-8°C and protect from light; reconstituted reagents should be aliquoted and frozen to limit freeze-thaw cycles across the shelf life.