Rabbit Ubiquitin (Ub) ELISA Kit

Quantitative measurement of Ubiquitin in rabbit-derived specimens is the focus of this colorimetric microplate-based immunoassay from Assay Genie. Ubiquitin (Ub) is a small, highly conserved 8.5 kDa regulatory protein present in virtually all eukaryotic cells, where it tags substrate proteins for 26S proteasome-mediated degradation, modulates receptor trafficking, and shapes DNA-damage responses. Altered Ub homeostasis is implicated in neurodegeneration, oncogenesis, and chronic inflammation, making accurate quantification a routine readout in cell biology, pharmacology, and disease-model research.

The kit is calibrated to accept a diverse panel of sample matrices derived from blood components, tissue homogenates, isolated-cell preparations, and conditioned media from cell culture systems, making it broadly applicable to in vitro studies of protein turnover, signalling pathway dissection, and biomarker tracking in rabbit models. Each kit ships with the reagents required to complete a 96-test workflow and includes detailed protocol guidance for both standard curve preparation and sample dilution. The assay is intended for research use only and supports laboratories working on ubiquitin-proteasome system function, post-translational modification analysis, and pathway-focused drug screening.

Storage and handling recommendations provided in the package insert should be observed to preserve antibody activity and standard integrity across runs. Researchers commonly pair this ELISA with downstream western blot validation to confirm ubiquitin conjugation states and substrate identities.