Rabbit Tumor Necrosis Factor Alpha (TNFa) ELISA Kit

Quantitative measurement of TNF-alpha in rabbit samples is enabled through an antibody-pair immunoassay, with a capture antibody pre-coated onto a microplate for reproducible cytokine quantification across inflammation, immunology, and biomedical research studies. Compatible input material includes cell lysates, tissue homogenates, cell culture supernatants, and blood-derived matrices such as serum or plasma, loaded at a working volume of 100 µL per well. The protocol uses a colorimetric readout and is intended for research use only. Reagents should be stored refrigerated at 4°C, while reconstituted standard vials should be frozen at −20°C and protected from repeated freeze-thaw cycles. Each Assay Genie kit supplies pre-coated wells, detection antibody, lyophilized standards, streptavidin-HRP conjugate, wash buffer, and substrate solution sufficient for one microplate run completed in approximately 4.5 hours.