Rabbit Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit

A quantitative immunoassay built for measuring Transforming Growth Factor Beta 1 in rabbit-derived samples. The kit supports research into TGF-β1 signaling pathways, which regulate fibroblast activity, extracellular matrix deposition, T-cell differentiation, and epithelial-mesenchymal transition. In rabbit models, TGF-β1 measurement is frequently used to study wound healing kinetics, scar formation, pulmonary fibrosis, hepatic injury responses, renal interstitial remodeling, and ocular tissue scarring following surgical or chemical insult.

The kit is supplied with a precoated microplate, biotinylated detection antibody, standard diluent, streptavidin-HRP conjugate, wash buffer concentrate, chromogenic substrate solution, and stop reagent. Researchers run samples alongside a recombinant TGF-β1 standard curve and read absorbance in a standard microplate reader. Standard curves are typically fit using four-parameter logistic regression to interpolate concentrations.

Storage and handling: reagents are stable when refrigerated until the expiration date printed on the label. For longer-term storage of working stocks, freeze aliquots of diluted standards and avoid repeated freeze-thaw cycles. Bring all kit components to room temperature before use.

Sample preparation from rabbit subjects typically yields serum along with platelet-poor plasma, tissue homogenates from lung, liver, kidney, heart, or skin, cell lysates from cultured primary cells, or cell culture supernatants from stimulated splenocytes or fibroblasts. The kit is configured for in vitro research use only and is not intended for diagnostic procedures in humans or animals.

Each Assay Genie kit contains sufficient reagents for one full plate. The accompanying protocol provides step-by-step guidance on standard preparation, sample dilution, incubation times, and wash procedures to support reproducible quantification of TGF-β1 across experiments.