Rabbit LIF DIY ELISA Kit

The Rabbit LIF DIY ELISA Kit from Assay Genie supports research on Leukemia Inhibitory Factor (LIF) in rabbit-derived experimental systems. LIF is a pleiotropic cytokine best known for sustaining pluripotency of mouse embryonic stem cells and for its role in blastocyst implantation, making quantitative measurement of LIF protein a common requirement in reproductive biology, stem cell maintenance, and inflammatory research.

This DIY format supplies the framework — pre-coated capture plates, diluents, wash buffer, substrate, and stop solution — while the end user supplies their own anti-LIF antibody pair and reference standard, allowing laboratories to adapt the workflow to proprietary antibodies or custom standard preparations. The kit is sized for Up to 10 Plates, accommodating both small pilot studies and larger cohort analyses of cell-culture supernatants, tissue homogenates, or serum from rabbit models.

Standard workflow uses a 100 µL sample volume per well with absorbance measured on a standard microplate reader. Unopened kit components should be stored refrigerated at 4 °C and protected from light to preserve reagent stability; working dilutions should be prepared fresh and not subjected to repeated freeze-thaw cycles. The single-use plate strips allow flexible plate usage without wasting reagents on partial runs, and the kit is intended for research use only.