Rabbit Interleukin 1 Receptor Antagonist (IL-1RA) ELISA Kit

A quantitative immunoassay from Assay Genie, this ELISA kit measures interleukin-1 receptor antagonist (IL-1RA) levels in rabbit-derived samples and supports research workflows in inflammation, autoimmunity, and cytokine signalling studies. The kit uses a pre-coated microplate and a colorimetric read-out protocol, accepting tissue homogenates, cell lysates, and cell culture supernatants without pre-treatment. Each box contains sufficient reagents to perform 96 individual determinations, making it appropriate for mid-throughput screening of treatment cohorts in rabbit models.

IL-1RA acts as a competitive antagonist at the interleukin-1 receptor, and tracking its concentration is a common readout in studies of acute-phase responses, vaccine adjuvant research, and chronic inflammatory disease models. Researchers can apply the kit to compare endogenous IL-1RA expression between control and challenged animal groups, evaluate recombinant antagonist production in transfected cell lines, or monitor baseline cytokine tone in longitudinal sampling studies. The workflow relies on standard laboratory instrumentation, including a microplate reader with absorbance capability, an automated or manual plate washer, and calibrated single- and multi-channel pipettes.

Reconstituted standard and detection antibody vials should be kept refrigerated between uses, and unused microplate strips returned to the foil pouch with desiccant to preserve binding activity. The kit is supplied with a lot-specific protocol insert that details sample dilution ranges, incubation times, and calculation guidance for interpolating unknown concentrations from the standard curve. This product is labelled for research use only and is not cleared for diagnostic applications.