Rabbit Interleukin 1 Beta (IL-1b) ELISA Kit

Interleukin-1 beta (IL-1β) is a key pro-inflammatory cytokine central to fever response, leukocyte recruitment, and acute phase signaling. Quantifying circulating IL-1β in rabbit models is widely used in inflammation, arthritis, sepsis, and vaccine response research. This Sandwich ELISA from Assay Genie is calibrated for rabbit-specific IL-1β capture, ensuring accurate quantification without significant cross-reactivity to rodent or human homologs.

The assay has a standard detection range of 15.6 to 1000 pg/mL with a sensitivity below 5.6 pg/mL, requiring a 100 μL sample volume per well. Pre-coated capture antibody wells are incubated with standards or samples, followed by a biotinylated detection antibody and streptavidin-HRP conjugate. The colorimetric reaction is read at 450 nm against a standard curve. Validated for serum, plasma, tissue homogenates, cell lysates, and cell culture supernatants, the kit supports in vitro and ex vivo research workflows.

Reagents should be refrigerated at 2-8°C; bring all components to room temperature before use to avoid condensation. The 96-well microplate format supports reproducible data for veterinary research, pharmacokinetic studies, and biomedical investigations of inflammatory pathways and cytokine biology.