Rabbit IL-6 DIY ELISA Kit

The Assay Genie Rabbit IL-6 DIY ELISA Kit ships with all reagents needed to coat and run up to 10 microplates, including a rabbit-specific capture antibody, biotinylated detection reagent, recombinant IL-6 standard, and streptavidin-HRP working solution. Researchers coat their own 96-well plates, which provides flexibility to scale experiments and adjust coating concentrations. Interleukin-6 (IL-6) is a pleiotropic cytokine involved in fever, acute-phase protein induction, and B-cell differentiation, so quantifying rabbit IL-6 supports studies of vaccine immunology, inflammatory disease models, and surgical recovery. Standard protocol uses a 100 µL sample volume per well with TMB substrate for color development, and absorbance is recorded after stop solution addition. Reconstituted standard should be aliquoted and stored frozen to limit freeze-thaw cycles, while other components are kept refrigerated at 4 °C.