Rabbit IL-4 ELISA Kit

From Assay Genie, this Sandwich ELISA Kit provides a sensitive double-antibody method for the quantitative detection of interleukin-4 (IL-4) in rabbit samples. The assay is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Performance specifications include a sensitivity of 9.375 pg/mL and a working range of 15.625-1000 pg/mL. The procedure uses a biotinylated detection antibody, an HRP-streptavidin conjugate, and TMB substrate, with absorbance read at 450 nm. Reported recovery averages 92% in serum, 91% in EDTA plasma, and 94% in heparin plasma, with linearity ranging from 80% to 105% across 1:2 to 1:8 dilutions. Unopened kits are stored at 2-8°C for 12 months.

IL-4 is a key cytokine involved in regulating immune responses, inflammation, and allergic reactions, supporting research into immune disorders, autoimmune diseases, and inflammatory conditions. The target is annotated under UniProt ID Q9MZR8.