Rabbit IL-1RA/IL-1 receptor alpha ELISA Kit

Assay Genie's Rabbit IL-1RA/IL-1 receptor alpha ELISA Kit is a sandwich-format, double-antibody assay designed for the quantitative measurement of IL-1RA/IL-1 receptor alpha in rabbit samples. The kit uses colorimetric detection and is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Performance specifications include a sensitivity of 18.75 pg/mL and a standard curve range of 31.25-2000 pg/mL. Reagents are stored at 2-8°C for up to 12 months. The workflow includes a 90-minute primary incubation at 37°C, a 60-minute biotin-labeled detection antibody step, 30 minutes of HRP-Streptavidin binding, TMB color development in the dark for 10-20 minutes, and absorbance reading at 450 nm after stop solution addition.

IL-1 receptor alpha is a key regulator of the inflammatory response, acting through modulation of interleukin-1 cytokine activity. The kit supports research into inflammatory and autoimmune disease mechanisms, and related fields of immunology and biomedicine.