Rabbit IL-1A/IL-1 alpha ELISA Kit

This Sandwich ELISA from Assay Genie provides quantitative measurement of IL-1A/IL-1 alpha in rabbit samples using a double-antibody format. The colorimetric assay delivers a sensitivity of 9.375 pg/mL across a working range of 15.625-1000 pg/mL and accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. IL-1A/IL-1 alpha is described as a key cytokine involved in inflammation and immune responses, relevant to research in autoimmune, infectious, and inflammatory disorders.

Validated linearity in serum, EDTA plasma, and heparin plasma at 1:2, 1:4, and 1:8 dilutions falls between 82% and 102%, while recovery across the same matrices averages between 92% and 100%. The kit is stored at 2-8°C for 12 months.

Color development uses TMB substrate with absorbance read at 450 nm, following primary incubation at 37°C for 90 minutes, biotin-labelled detection antibody binding for 60 minutes, and HRP-Streptavidin (SABC) incubation for 30 minutes.