Rabbit IL-10 ELISA Kit

From Assay Genie, this colorimetric ELISA Kit is built on a sandwich ELISA format using a double-antibody method, with absorbance read at 450 nm. Reactive with rabbit samples, the assay has been validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and the reagents are stored at 2–8°C for 12 months.

The assay achieves a sensitivity of 9.375 pg/mL and a working range of 15.625–1000 pg/mL. Recovery in spiked samples returned 91–101% (average 96%) for serum, 86–103% (average 96%) for EDTA plasma, and 85–104% (average 95%) for heparin plasma. Linearity was confirmed at 1:2, 1:4, and 1:8 dilutions, with ranges of 83–101% for serum, 81–100% for EDTA plasma, and 83–100% for heparin plasma. The kit targets IL-10 (UniProt Q9TSJ4), also referred to as Interleukin 10, IL10, IL10A, CSIF, or TGIF, and is supplied in a solid-phase format.