Rabbit GSN/Gelsolin ELISA Kit

Designed for research use, this Sandwich ELISA Kit from Assay Genie enables accurate quantification of gelsolin (GSN) in rabbit samples. It is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Gelsolin is a key protein involved in regulating actin dynamics and cell motility, playing a crucial role in processes such as cell migration, adhesion, and signalling. Dysregulation of gelsolin has been linked to various diseases, including cancer, inflammation, and neurodegenerative disorders, highlighting its importance as a biomarker for studying these conditions.

The assay is configured as a Sandwich ELISA with double-antibody detection. Reported sensitivity is 3.75 ng/mL, with a working range of 6.25–400 ng/mL. The protocol involves equilibrating reagents and TMB substrate to room temperature, primary incubation at 37°C, sequential addition of a biotin-labelled detection antibody and HRP-Streptavidin (SABC), colour development with TMB substrate in the dark, and a stop reaction with absorbance measured at 450 nm.

Reactivity is specific to rabbit samples, and the kit is intended for storage at 2–8°C for 12 months. Listed synonyms include GS, Gelsolin, GSN, ADF, AGEL, Brevin, and Actin-depolymerizing factor, with the associated UniProt identifier Q0GA40.