Rabbit Apolipoprotein CI/APOC1 ELISA Kit

Designed for the quantitative measurement of Apolipoprotein CI (APOC1) in rabbit samples, this Sandwich ELISA kit from Assay Genie uses a double-antibody, colorimetric detection method. The assay is reactive with rabbit and accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types, making it suitable for research into lipid metabolism, cardiovascular disease, atherosclerosis, and hyperlipidemia in rabbit model systems. The associated UniProt ID is P33047, with synonyms including Apo-C1, Apolipoprotein C1, Apolipoprotein C-I, APO-C1, and Apo-C1.

Linearity (n=5) is 91-99% at 1:2, 93-105% at 1:4, and 91-99% at 1:8 for serum; 85-101%, 85-101%, and 82-97% for EDTA plasma; and 80-98%, 95-100%, and 86-98% for heparin plasma. Recovery (n=5) is 90-105% (average 96%) for serum, 85-101% (average 94%) for EDTA plasma, and 94-103% (average 99%) for heparin plasma.

The assay procedure involves equilibrating reagents and TMB substrate to room temperature, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding at 37°C for 60 minutes, HRP-Streptavidin incubation at 37°C for 30 minutes, TMB color development in the dark for 10-20 minutes, and absorbance measurement at 450 nm after stop solution addition. The format is solid phase.