Rabbit Apolipoprotein B/ApoB ELISA Kit

This Sandwich ELISA kit from Assay Genie is designed for the quantitative measurement of Apolipoprotein B (APOB) in rabbit serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay uses a double-antibody sandwich format with colorimetric detection, and targets a protein that is central to lipid metabolism and is implicated in cardiovascular disease, atherosclerosis, and other metabolic disorders. Monitoring APOB levels with this kit can support research into the mechanisms of these conditions and potential therapeutic interventions.

Reported performance characteristics include a sensitivity of 46.875 ng/mL and a measuring range of 78.125–5000 ng/mL. Unopened kits are stored at 2–8 °C for 12 months. The kit lists synonyms ApoB100, Apolipoprotein B100, and Apo-B100, and is mapped to UniProt Q7M2U9.

Validation data include linearity and recovery across serum, EDTA plasma, and heparin plasma. Linearity in serum (n=5) ranged from 92–105% at 1:2, 89–105% at 1:4, and 85–104% at 1:8; EDTA plasma (n=5) showed 88–101%, 90–101%, and 87–95% at the same dilutions; and heparin plasma (n=5) showed 80–100%, 81–94%, and 81–95%. Recovery ranges were 89–102% for serum (average 95%), 89–105% for EDTA plasma (average 100%), and 86–96% for heparin plasma (average 91%).