Rabbit Angiopoietin-1 PharmaGenie ELISA Kit (SBRS1246)

The Rabbit Angiopoietin-1 PharmaGenie ELISA Kit from Assay Genie is configured as a 96-assay microplate kit that uses a matched-antibody capture format with an absorbance-based readout, supporting vascular biology and angiogenesis research programs. Angiopoietin-1 (Ang-1) is the principal Tie2 receptor ligand that drives vessel maturation, pericyte recruitment, and endothelial barrier stabilization, so quantitative measurement of circulating Ang-1 concentrations is widely used to track neovascularization, wound healing, tumor microvascular remodeling, and experimental models of vascular leakage.

The kit accepts cell culture supernatants and rabbit-derived liquid samples with a recommended 100 µL sample volume per well. Each shipment from Assay Genie includes pre-coated capture wells, biotinylated detection antibody, recombinant Ang-1 standards, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop reagent, so laboratories can complete a full quantitative run within a single working day using standard plate-reader equipment.

Researchers commonly pair Ang-1 measurements generated with this kit with VEGF, FGF-2, and Tie2 expression data to characterize angiogenic signaling networks in rabbit ocular, ischemic limb, and tumor implantation models. Best-practice recommendations include running all standards and unknowns in duplicate, equilibrating frozen aliquots to room temperature before loading, and processing plasma or serum fractions in parallel to limit matrix-related drift between Ang-1 concentrations.