QuickStep Sheep Cortisol ELISA Kit

Quantitative measurement of cortisol in ovine samples using a competitive immunoassay format. Cortisol, the primary glucocorticoid synthesized by the adrenal cortex, serves as a key biomarker in veterinary endocrinology research. This competitive ELISA from Assay Genie is calibrated for sheep specimens, with the microplate pre-coated with a cortisol conjugate and detection based on a labeled anti-cortisol antibody competing with free cortisol in standards and unknowns.

Applications include stress physiology studies covering transport, weaning, handling, and husbandry stress responses, reproductive endocrinology around lambing, lactation, and seasonal breeding cycles, welfare assessment in production flocks, and metabolic or nutritional research. The microplate format supports moderate-throughput screening in a single run, and colorimetric readout makes the protocol compatible with standard plate readers found in veterinary and animal science laboratories.

Sample handling: collect blood into appropriate tubes for serum or plasma preparation, allow clotting where required for serum, centrifuge, aliquot, and store refrigerated or frozen if not assayed immediately. Avoid repeated freeze-thaw cycles by freezing single-use aliquots. The kit supplies pre-coated wells, cortisol standards, detection antibody, and substrate sufficient for the full plate run. Bring all reagents to room temperature before use and protect light-sensitive components from direct exposure during the incubation steps.