QuickStep Mouse E3 (Estriol) ELISA Kit

A competitive immunoassay from Assay Genie, the QuickStep Mouse E3 ELISA Kit quantitatively measures estriol in mouse research samples. Estriol (E3) is one of the three principal estrogens and is of particular interest in reproductive biology, gestation studies, and endocrine research using murine models, where its circulating profile differs from estradiol and estrone.

Each kit is configured as a 96 Assays microplate strip format, providing capacity for full standard curves and experimental replicates within a single run. The protocol uses an absorbance-based readout on a standard plate reader and is calibrated specifically for mouse reactivity rather than adapted from a human or rat protocol.

Researchers commonly apply this kit to assess estrogen dynamics in rodent pregnancy models, ovariectomy studies, and hormone replacement experiments, where estriol serves as a marker of fetoplacental wellbeing and steroid metabolism. The competitive design is well matched to small unconjugated steroid analytes like estriol that circulate at low concentrations and compete with labelled antigen for antibody binding sites.

Kit contents include a pre-coated microplate, estriol standards, detection reagents, wash buffer, and substrate, supplied in individual vials sized for the 96 Assays run. Store unopened kit components at the temperature indicated on the product insert and allow all reagents to equilibrate to room temperature before use.