PYK2 (Phospho-Tyr579)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA Kit is designed for the precise measurement of phosphorylated PYK2 at tyrosine 579 in cells, using a colorimetric detection method at 450 nm. The assay is reactive with Human, Mouse, and Rat samples and is intended for use with adherent cells. It provides a convenient, lysate-free, high-throughput workflow suitable for detecting PYK2 protein phosphorylation and expression profiles in cultured cells, as well as screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on PYK2 phosphorylation.

Qualitative determination is achieved via an indirect ELISA format. PYK2 (Phospho-Tyr579)-specific primary antibodies capture the target, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, allowing the conjugated HRP to catalyze a colorimetric reaction upon substrate addition. Because the assay is qualitative, two normalisation methods are required: a monoclonal antibody specific for human GAPDH is included as an internal positive control, and a Crystal Violet whole-cell staining method is provided to determine cell density so that absorbance values can be adjusted for plating differences.

The target, PYK2 (also known as protein-tyrosine kinase 2-beta), is a nonreceptor tyrosine kinase of the Fak family encoded by the gene PTK2B (UniProt ID: Q14289, OMIM: 601212). It is predominantly expressed in cells derived from hematopoietic lineages and in the central nervous system and plays a role in cell signalling, adhesion, and migration. Dysregulation of PYK2 phosphorylation has been implicated in cancer, inflammatory disorders, and neurodegenerative conditions, supporting its relevance to basic research and therapeutic development. The stated dynamic range is > 5000 Cells.