PYK2 Colorimetric Cell-Based ELISA Kit

Manufactured by Assay Genie, this Cell-Based ELISA is a convenient, lysate-free, high-throughput assay designed to detect PYK2 protein expression in cultured cells. The kit is reactive with Human, Mouse, and Rat samples and is intended for use with adherent cells. Detection is colorimetric at 450 nm, with a quoted dynamic range of > 5000 Cells, and the kit is stored at 4°C / 6 Months.

The assay uses an indirect ELISA format in which PYK2-specific primary antibodies capture the target and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody; the conjugated HRP then catalyses a colour reaction upon substrate addition. Because the Cell-Based ELISA is qualitative, two normalisation approaches are provided: a monoclonal antibody specific for human GAPDH acts as an internal positive control for normalising target absorbance, and Crystal Violet whole-cell staining is used to determine cell density for further normalisation of plating differences.

The kit can be used to measure relative PYK2 levels in cultured cells and to screen the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on PYK2. PYK2 (gene symbol PTK2B; Gene ID 2185; UniProt Q14289; chromosomal location 8p21.1) is a non-receptor tyrosine kinase of the Fak family, predominantly expressed in cells derived from hematopoietic lineages and in the central nervous system, where it participates in cell adhesion, migration, calcium-induced regulation of ion channels, and activation of the MAP kinase signalling pathway.