PTEN (Phospho-Ser380/Thr382/Thr383)Colorimetric Cell-Based ELISA Kit

Designed for the accurate detection of PTEN phosphorylation at serine 380, threonine 382, and threonine 383, this cell-based ELISA kit from Assay Genie provides a sensitive, high-throughput, lysate-free workflow for adherent cell samples. The assay is reactive with human, mouse, and rat specimens and uses a colorimetric detection method read at 450 nm, with a dynamic range of > 5000 Cells.

The kit employs an indirect ELISA format in which phosphorylation site-specific primary antibodies capture PTEN, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody to catalyze a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values. Following the colorimetric readout, the Crystal Violet whole-cell staining method may be used to determine cell density, allowing absorbance values to be normalized to cell amounts to adjust for plating differences.

PTEN is a critical tumor suppressor that regulates cell growth, apoptosis, and cell cycle progression, and phosphorylation at these sites can modulate its enzymatic activity and protein stability. The kit can be used to measure relative levels of phosphorylated PTEN in cultured cells or to screen the effects of treatments, inhibitors such as siRNA or chemicals, or activators on PTEN phosphorylation, making it a valuable tool for investigating PTEN signaling in cancer and other diseases.