PTEN (Phospho-Ser370)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA Kit is designed for the accurate measurement of PTEN phosphorylation at serine 370 in cell lysates. The assay offers high sensitivity and specificity, providing precise and reliable results across a variety of research applications, including studies of cancer biology, signalling pathways and the identification of potential therapeutic targets.

Using a lysate-free, indirect ELISA format, the kit captures Phospho-PTEN (Ser370) with specific primary antibodies and then detects bound target with HRP-conjugated secondary antibodies that bind the Fc region of the primary antibody. A colour reaction is generated through HRP-catalysed substrate conversion and is read colorimetrically at 450 nm. Relative levels of phosphorylated PTEN can be assessed in cultured cells, and the workflow supports screening the effects of treatments, inhibitors (such as siRNA or chemicals) or activators on PTEN phosphorylation. A monoclonal GAPDH antibody is included as an internal positive control for normalising target absorbance values, and Crystal Violet whole-cell staining may be used to determine cell density for further normalisation.

Reactivity is reported for human, mouse and rat samples, with a dynamic range of > 5000 cells and a sample type of adherent cells. PTEN (phosphatase and tensin homolog) is a critical tumour suppressor gene that regulates cell growth and division, and phosphorylation at serine 370 can influence its tumour suppressor function. PTEN is a phosphoinositide 3-phosphatase (PIP3) and a major negative regulator of the PI3K/Akt signalling pathway, possessing a C-terminal regulatory domain that contains three phosphorylation sites which may regulate its stability. Associated database identifiers are Gene ID 5728 and UniProt ID P60484.