Prostate Apoptosis Response protein-4 Colorimetric Cell-Based ELISA Kit

A Cell-Based ELISA Kit from Assay Genie designed for the detection of Prostate Apoptosis Response protein-4 in cells. The assay is described as a convenient, lysate-free, high-throughput and sensitive format that uses an indirect ELISA approach: the target is captured by PAWR-specific primary antibodies, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody to drive a colorimetric reaction read at 450 nm. The kit is intended for use with adherent cells and shows reactivity with human, mouse and rat samples, with a stated dynamic range of "> 5000 Cells" and storage at 4 °C for 6 months.

For data normalisation, the kit includes a monoclonal anti-GAPDH antibody that serves as an internal positive control, alongside a Crystal Violet whole-cell staining method that can be used to determine cell density and correct absorbance readings for plating differences. PAWR (also known as Par-4 / Prostate apoptosis response 4 protein) is a proapoptotic protein linked to cancer cell apoptosis and tumour suppression, supporting the kit's relevance to apoptosis and oncology research.