PPP1R2 Colorimetric Cell-Based ELISA Kit

Designed for accurate measurement of PPP1R2 levels in cell lysates and tissue homogenates, this cell-based ELISA kit from Assay Genie provides a sensitive, lysate-free, high-throughput workflow for profiling PPP1R2 protein expression in cultured cells. The kit can be used to screen the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on PPP1R2, and is described in the product overview as suitable for measuring PPP1R2 in serum, plasma, and lysate samples.

Qualitative determination of PPP1R2 is achieved through an indirect ELISA format: PPP1R2-specific primary antibodies capture the target, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction upon substrate addition. A monoclonal anti-GAPDH antibody is included to serve as an internal positive control in normalising the target absorbance values, and the Crystal Violet whole-cell staining method may be used to determine cell density. The assay is read at 450 nm.

The kit is reactive with Human, Mouse, and Rat samples, uses a colorimetric detection method, and has a dynamic range of > 5000 Cells. It is supplied in a microplate format, stored at 4°C for 6 months, and is intended for use with adherent cells. The target, PPP1R2 (also known as protein phosphatase 1 regulatory subunit 2), is a member of the protein phosphatase inhibitor 1 family; its gene is located at chromosomal region 3q29, encodes a protein of 23,015 Da (UniProt P41236; NCBI Gene ID 5504), and two alternatively-spliced isoforms have been described.