PPP1R14C Colorimetric Cell-Based ELISA

From Assay Genie, this Colorimetric Cell-Based ELISA Kit provides a sensitive tool for the detection and quantification of PPP1R14C levels in cell lysates and tissue extracts. The assay uses an indirect ELISA format in which PPP1R14C is captured by target-specific primary antibodies, with HRP-conjugated secondary antibodies binding the Fc region of the primary antibody. A colorimetric reaction is produced upon substrate addition, with absorbance read at 450 nm, allowing researchers to measure relative protein expression in cultured cells and to screen the effects of various treatments, inhibitors, or activators.

The kit is designed for use with adherent cells and shows reactivity with human, mouse, and rat samples. It supports a dynamic range of greater than 5000 cells. For data normalization, a monoclonal GAPDH antibody is included as an internal positive control, and a Crystal Violet whole-cell staining method can be used to determine cell density so that absorbance values can be normalized to cell number and plating differences adjusted. Storage is at 4°C with a stability of 6 months.

PPP1R14C (also known as KEPI) is a key regulatory protein involved in cellular processes such as cell division and migration. Its dysregulation has been linked to cancer and neurodegenerative disorders, making it a valuable biomarker for studying cellular function and disease progression.