PPAR-gamma Colorimetric Cell-Based ELISA Kit

Designed to detect PPAR-gamma (peroxisome proliferator-activated receptor gamma) protein expression in cultured cells, this Colorimetric Cell-Based ELISA Kit from Assay Genie uses an indirect ELISA format in which PPAR-gamma-specific primary antibodies capture the target, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody to catalyse a colorimetric reaction upon substrate addition.

Described as a convenient, lysate-free, high-throughput and sensitive assay, the kit can be used to measure relative amounts of PPAR-gamma in cultured adherent cells and to screen the effects of various treatments, inhibitors (such as siRNA or chemicals) or activators on PPAR-gamma. Detection is colorimetric, measured at 450 nm, with a 96-Well Microplate format and a dynamic range of > 5000 Cells. Reactivity is reported for Human, Mouse and Rat samples. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control for normalising target absorbance values, and a Crystal Violet whole-cell staining step may be used to determine cell density for further normalisation.

PPAR-gamma (gene symbol PPARG) is a nuclear receptor involved in regulating gene expression and is known to play a critical role in metabolic processes including lipid metabolism and insulin sensitivity, with a reported molecular weight of 59.2kD. The kit is supplied for storage at 4°C with a stability of 6 Months and is intended for research applications in cell biology, including studies of metabolic disorders such as obesity, diabetes and cardiovascular diseases.