POU4F1 Transcription Factor Activity Assay

The POU4F1 Transcription Factor Activity Assay from Assay Genie is a DNA-binding immunoassay that quantifies the binding activity of the POU4F1 (BRN3A) transcription factor in nuclear and cell lysates. POU4F1 is a class IV POU domain factor critical for neuronal differentiation, retinal ganglion cell development, and sensory neuron survival, and is also implicated in melanoma and neuroblastoma pathways. This 96 Assays microplate format supports research into neurogenesis, apoptosis regulation, and cancer biomarker studies across human, mouse, and rat samples. The procedure uses a colorimetric read-out in a microplate layout and is intended for research use only in vitro applications. Store kit components at -20 degrees C in single-use aliquots to preserve reagent activity and avoid repeated freeze-thaw cycles before each assay run.