Porcine Zona pellucida sperm-binding protein 3 (ZP3) ELISA Kit

The Assay Genie Porcine ZP3 ELISA Kit quantifies zona pellucida sperm-binding protein 3 in porcine serum and plasma samples using a sandwich ELISA format. ZP3 is a glycoprotein component of the egg's extracellular coat and mediates species-specific sperm recognition during fertilization, making this kit useful for reproductive biology research, oocyte maturation studies, and investigations into ovarian function.

Each kit contains a pre-coated 96-well microplate, standards, detection antibodies, and reagents for colorimetric readout at 450 nm. The assay typically requires 100 ul of sample per well and follows a standard incubation protocol. Reagents should be stored at 2-8 degrees C and the standard kept frozen at -20 degrees C until use to preserve activity.

Researchers studying boar fertility, sperm-egg binding mechanisms, or zona pellucida glycoprotein expression in swine reproductive models can use this kit as a quantitative tool. The assay is configured for research use only and is not intended for diagnostic applications.