Porcine WAP four-disulfide core domain protein 2 (WFDC2) ELISA Kit

The Assay Genie Porcine WFDC2 ELISA Kit uses a quantitative sandwich ELISA configuration in which a pre-coated capture antibody immobilized on a 96-well microplate binds WFDC2 from porcine serum or plasma samples. A biotinylated detection antibody combined with a streptavidin-HRP conjugate generates a colorimetric signal measurable at 450 nm, with absorbance intensity proportional to WFDC2 concentration. Each kit ships with a recombinant standard curve for accurate interpolation, and the protocol typically requires only 100 µl of sample per well.

WFDC2 (WAP four-disulfide core domain protein 2) is a secreted small-proteinase inhibitor belonging to the WAP domain family, and is investigated in porcine reproductive biology, mucosal immunity, and inflammatory biomarker studies. Researchers use this in vitro assay to quantify endogenous WFDC2 levels in porcine serum or plasma, supporting work in swine health, infectious disease models, and comparative biomarker discovery across mammalian species.

Unopened kits should be stored at 2-8°C, avoiding freeze-thaw cycles; reconstituted standards and unused strip wells are best kept at -20°C to preserve antibody activity. Intended for research use only and not validated for diagnostic procedures.