Porcine Type II iodothyronine deiodinase (DIO2) ELISA Kit

The Assay Genie Porcine Type II iodothyronine deiodinase (DIO2) ELISA Kit uses a sandwich immunoassay principle to quantitatively measure DIO2 protein levels in porcine serum and plasma samples. DIO2 is a selenocysteine-containing enzyme that catalyzes outer-ring deiodination of thyroxine (T4) into bioactive 3,3',5-triiodothyronine (T3), and it plays a central role in local thyroid hormone activation within tissues such as the brain, brown adipose tissue, and skeletal muscle.

Each kit is supplied as a 96-well microplate format containing all reagents required to generate a standard curve and quantify unknown samples by colorimetric detection read at 450 nm. The assay is intended for research use only in studies of porcine metabolism, endocrine function, and comparative endocrinology. Reconstituted standards and undiluted samples are typically stored at 2 to 8 degrees C, with longer-term storage at -20 degrees C recommended for working aliquots to minimize freeze-thaw cycles.

Validation against porcine-specific samples delivers reliable intra-assay and inter-assay precision, supporting reproducible quantification across experimental runs. Investigators working with swine disease models in metabolic disorders, obesity, and thermoregulation can incorporate this ELISA to monitor DIO2 expression, protein abundance, and tissue-level activity in circulation-derived specimens.