Porcine TIMP1/Metalloproteinase inhibitor 1 ELISA Kit

A colorimetric Sandwich ELISA with a double-antibody format from Assay Genie, designed for the precise measurement of TIMP1 (Metalloproteinase Inhibitor 1) levels in porcine samples. The kit accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and is intended to be stored at 2-8°C for 12 months.

The assay delivers a sensitivity of 0.188 ng/mL across a working range of 0.313-20 ng/mL. Reported linearity across 1:2, 1:4, and 1:8 dilutions runs 81-105%, 82-95%, and 80-98% for serum (n=5); 87-105%, 86-100%, and 80-97% for EDTA plasma (n=5); and 85-104%, 81-98%, and 87-99% for heparin plasma (n=5). Recovery ranges from 82-93% (average 87%) in serum, 80-99% (average 90%) in EDTA plasma, and 80-97% (average 89%) in heparin plasma.

TIMP1 is a critical regulator of matrix metalloproteinases (MMPs), playing a vital role in tissue remodeling, wound healing, and inflammation. Abnormal TIMP1 levels have been linked to diseases including cancer, fibrosis, and cardiovascular disorders, making the kit a valuable biomarker research tool. The associated Uniprot identifier is P35624, with synonyms including CLGI, Collagenase inhibitor, EPA, EPO, HCI, Metalloproteinase inhibitor 1, TIMP, TIMP 1, and TIMP1.