Porcine T4 (Thyroxine) ELISA Kit (PRFI00228)

The Assay Genie porcine thyroxine immunoassay uses a competitive inhibition format in which sample T4 competes with coated antigen for a labelled antibody, producing an inverse absorbance signal quantified against a calibrated standard curve. Each reaction requires 50 µL of sample, and the full microplate can be processed within a standard working day once standards and enzyme conjugate are loaded. Reconstituted reagents remain stable through multiple freeze-thaw cycles when aliquoted, and the pre-coated strip plate should be kept sealed with desiccant until use. Researchers commonly apply this porcine-specific T4 assay to endocrinology, nutrition, and reproduction studies in swine, where circulating thyroxine is used as a biomarker for metabolic status, growth performance, and gestational development.