Porcine Resistin ELISA Kit

Assay Genie supplies a sandwich double-antibody ELISA designed to quantify resistin in porcine serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with a colorimetric readout based on TMB substrate development and absorbance measurement at 450 nm.

The reported sensitivity is 37.5 pg/mL and the assay range is 62.5-4000 pg/mL. Linearity at 1:2, 1:4 and 1:8 dilutions (n=5) is 85-102%, 84-101% and 82-100% for serum; 87-100%, 87-99% and 83-99% for EDTA plasma; and 88-103%, 83-90% and 92-100% for heparin plasma. Recovery ranges are 85-105% (average 99%) for serum, 85-98% (average 92%) for EDTA plasma, and 94-98% (average 97%) for heparin plasma. The kit is stored at 2-8°C for 12 months. Resistin (synonyms RETN, ADSF, Adipose tissue-specific secretory factor, HXCP1, RETN1, RSTNXCP1; Uniprot Q6QR67) is described as a key adipokine implicated in regulating glucose metabolism and insulin sensitivity, with elevated levels associated with obesity, insulin resistance and metabolic disorders.