Porcine REN (Renin) ELISA Kit

Manufactured by Assay Genie, this Porcine REN (Renin) ELISA Kit is a sandwich ELISA based on a double antibody configuration with a colorimetric readout, intended to quantify renin in porcine-derived samples. Renin is a key enzyme of the renin-angiotensin system with established roles in blood pressure regulation and electrolyte balance, and its dysregulation has been linked to various cardiovascular diseases.

Performance characteristics include a sensitivity of 46.875 pg/mL and a standard range of 78.125-5000 pg/mL. Validated sample types are serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Reported linearity for serum (n=5) is 86-103% at 1:2, 93-104% at 1:4, and 89-105% at 1:8; for EDTA plasma (n=5), 83-101%, 82-100%, and 88-98%; and for heparin plasma (n=5), 84-95%, 88-100%, and 80-91%. Recovery averages 94% for serum (range 85-102%), 95% for EDTA plasma (range 88-102%), and 93% for heparin plasma (range 85-97%). The workflow involves equilibrating reagents to room temperature, a primary incubation at 37°C for 90 minutes, detection antibody binding at 37°C for 60 minutes, HRP-streptavidin binding at 37°C for 30 minutes, TMB colour development in the dark for 10-20 minutes, and absorbance measurement at 450 nm after stop solution addition.

Supplied components include a dismountable ELISA microplate, lyophilized standard, biotin-labeled antibody (100X), HRP-streptavidin conjugate (SABC, 100X), TMB substrate, sample dilution buffer, antibody dilution buffer, SABC dilution buffer, stop solution, wash buffer (25X), plate sealer, and a technical manual. The kit is stored at 2-8°C for 12 months, with the biotin-labeled antibody, HRP-streptavidin conjugate, and TMB substrate kept away from direct light.