Porcine Progesterone ELISA Kit

From Assay Genie, this specialized immunoassay is designed for the precise quantification of progesterone in porcine samples. It supports research in reproductive biology and animal health, where monitoring progesterone — a hormone involved in regulating the estrous cycle and supporting pregnancy in pigs — is relevant to understanding reproductive function, determining the timing of ovulation, and diagnosing pregnancy-related disorders.

The assay has a stated sensitivity of 0.188 ng/mL and a detection range of 0.313–20 ng/mL. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit should be stored sealed at 2–8 °C and not cryopreserved. The procedure uses a biotin-labeled antibody and HRP-streptavidin conjugate with TMB substrate, with absorbance read at 450 nm on a microplate reader.

Linearity has been characterised at 1:2, 1:4 and 1:8 dilutions: 90–105%, 83–98% and 82–94% for serum (n=10); 85–101%, 83–98% and 80–93% for EDTA plasma (n=10); and 87–103%, 84–98% and 87–99% for heparin plasma (n=10). Recovery ranges are 86–100% (average 91%) for serum, 92–104% (average 99%) for EDTA plasma, and 93–102% (average 96%) for heparin plasma.