Porcine Pepsin ELISA Kit

Porcine pepsin is an aspartic protease secreted by chief cells of the gastric mucosa, where it initiates protein digestion by cleaving peptide bonds adjacent to aromatic amino acids. This ELISA kit from Assay Genie provides a quantitative tool for investigating digestive enzyme activity in swine models, supporting studies of gastric physiology, mucosal barrier function, and gastrointestinal disease mechanisms.

The assay follows an immunoassay workflow: a capture antibody coated on a microplate binds pepsin from prepared samples, a conjugated detection antibody binds the captured target, and an enzymatic substrate reaction generates a measurable signal proportional to analyte concentration. The resulting absorbance is read on a standard plate reader. Each kit contains pre-coated wells, assay buffers, detection reagents, lyophilised standards for curve generation, wash concentrate, and a stop solution, supplied in a single-use format sized for 96 individual determinations.

For research use only. Store kit components refrigerated between uses to maintain reagent stability. Intended for in vitro laboratory investigation of porcine gastrointestinal biology and enzyme regulation.