Porcine Nuclear receptor coactivator 1 (NCOA1) ELISA Kit

This Sandwich ELISA Kit from Assay Genie quantitatively measures porcine Nuclear receptor coactivator 1 (NCOA1), also known as SRC-1, a transcriptional coactivator that mediates ligand-dependent transactivation of steroid hormone receptors, thyroid hormone receptors, and retinoid receptors. The assay uses a capture antibody pre-coated on a 96-well microplate, paired with a biotinylated detection antibody and streptavidin-HRP, producing a colorimetric signal readable at 450 nm. Serum and plasma samples are validated for use, with results calibrated against a recombinant NCOA1 standard curve for accurate quantification across the dynamic range. Standard laboratory handling applies: store unopened kits at 2-8 °C, avoid freeze-thaw cycles of the reconstituted standard, and bring all reagents to room temperature before use. Intended for research use only, this kit supports studies in endocrinology, reproductive biology, and metabolic disease research where porcine models are relevant to translational investigation.

Target biology and biochemical function

NCOA1 (EC 2.3.1.48) is a 1440-residue nuclear protein of the p160 coactivator family that is highly conserved across mammals; the porcine ortholog shares approximately 93.73% cDNA identity with the human and mouse sequences. Beyond steroid receptor binding, the protein contains intrinsic histone acetyltransferase activity toward H3 and H4, and serves as an adaptor that recruits CBP and p300 to chromatin. It is required together with NCOA2 to balance white and brown adipose tissue activity, and it is essential for adipogenesis, making it a relevant readout in metabolic disorder research.

Typical workflow and performance

The complete sandwich ELISA workflow takes around 270 minutes at room temperature. Sample volume is 100 µL per well, with neat samples or 1:2 to 1:4 dilutions recommended for porcine serum and plasma. Reported reproducibility is strong, with intra-assay CV below 10% and inter-assay CV below 10%, and the standard curve typically spans 0.156 ng/mL to 10 ng/mL. Once received, the standard, Detection Reagent A, Detection Reagent B, and the 96-well strip plate should be stored at -20 °C, while all other components are kept at 2-8 °C.

Signaling context and translational value

NCOA1 participates in the intracellular steroid hormone receptor signaling pathway, nuclear hormone receptor binding, chromatin binding, and the regulation of lipid metabolism by PPARα. Because it directly coactivates estrogen receptor, androgen receptor, progesterone receptor, thyroid hormone receptor, retinoic acid receptor, and PPARγ signaling, the assay is a useful biomarker for endocrine, reproductive, and metabolic investigations in the pig model, and findings translate naturally to human studies of hormone-sensitive cancers, polycystic ovary syndrome, and thyroid disorders.