Porcine NT-ProANP (N-Terminal Pro Atrial Natriuretic Peptide) ELISA Kit

Assay Genie supplies a Sandwich ELISA, Double Antibody kit intended for the precise measurement of N-Terminal Pro Atrial Natriuretic Peptide (NT-ProANP) levels in porcine samples, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples [S1]. NT-ProANP is described as a critical peptide involved in regulating blood pressure and fluid balance, with relevance to cardiovascular conditions such as hypertension and heart failure, and the kit is presented as a tool for investigating cardiovascular function and related disorders in porcine models [S1].

Reported analytical performance includes a sensitivity of 0.188 ng/mL and an assay range of 0.313-20 ng/mL [S1]. Linearity across dilutions (1:2, 1:4, 1:8; n=5) is given as 85-105%, 88-99% and 81-99% for serum; 87-104%, 87-96% and 83-99% for EDTA plasma; and 86-102%, 82-101% and 82-94% for heparin plasma [S1]. Spike-and-recovery values (n=5) are 93-103% (average 99%) for serum, 89-104% (average 99%) for EDTA plasma, and 86-104% (average 94%) for heparin plasma [S1]. The associated Uniprot reference listed is P24259, and the synonym given is NT-proANP [S1].

The assay is performed on a pre-coated dismountable microplate using a biotin-labelled detection antibody and HRP-Streptavidin (SABC) detection chemistry, with TMB substrate development and absorbance reading at 450 nm after addition of stop solution [S1]. The kit is stored at 2-8°C and is stated to remain stable for 12 months under those conditions [S1].