Porcine NGF/Nerve Growth Factor ELISA Kit

From Assay Genie, this Sandwich ELISA kit uses a double-antibody format for the colorimetric detection of Nerve Growth Factor (NGF) in porcine samples. The assay is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, supporting a broad range of neuroscience and biomarker research applications.

Performance specifications include a sensitivity of 46.875 pg/mL and a working range of 78.125-5000 pg/mL. Recovery in serum (n=5) is reported as 89-100% with an average of 95%, in EDTA plasma (n=5) as 86-105% with an average of 96%, and in heparin plasma (n=5) as 91-105% with an average of 99%. Linearity data for serum, EDTA plasma, and heparin plasma across dilutions of 1:2, 1:4, and 1:8 are also provided. NGF is a key protein involved in neuronal growth and survival, and its dysregulation has been linked to neurodegenerative disorders, making it a valuable biomarker in related research.

The assay uses a biotin-labeled detection antibody followed by HRP-Streptavidin (SABC) binding, with TMB substrate development and absorbance reading at 450 nm. Reagents are stored at 2-8°C for up to 12 months.