Porcine MMP1 ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA kit is designed for the accurate and reliable measurement of MMP1 (Matrix Metalloproteinase 1) levels in porcine samples. MMP1 is a key enzyme involved in the breakdown of extracellular matrix proteins, playing a crucial role in tissue remodeling, wound healing, and inflammatory processes. Dysregulation of MMP1 activity has been linked to pathological conditions including arthritis, cancer metastasis, and cardiovascular diseases, making the kit a valuable research tool for studying MMP1 in disease contexts and identifying potential therapeutic targets.

Detection is colorimetric and the assay uses a double-antibody sandwich configuration. The kit reports a sensitivity of 0.469 ng/mL and a detection range of 0.781-50 ng/mL. Compatible sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit should be stored at 2-8°C for 12 months.

Validated linearity and recovery data are provided across serum, EDTA plasma, and heparin plasma. Serum samples (n = 5) show linearity of 82-85%, 83-88%, and 81-100% at 1:2, 1:4, and 1:8 dilutions respectively, with recovery ranging from 85-104% (average 93%). EDTA plasma (n = 5) shows linearity of 85-98%, 81-97%, and 85-102% at the same dilutions, with recovery of 90-101% (average 97%). Heparin plasma (n = 5) shows linearity of 82-96%, 91-101%, and 82-98%, with recovery of 89-98% (average 94%).