Porcine Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit

The Porcine Macrophage Inflammatory Protein 1 Beta (MIP-1b) ELISA Kit from Assay Genie targets CCL4, a beta-chemokine released during inflammatory responses in swine. Research applications include quantifying chemokine secretion in models of respiratory infection, sepsis, vaccine adjuvant response, and allograft rejection, where CCL4 serves as a biomarker of macrophage and lymphocyte activation.

The kit provides a precoated microplate, lyophilized recombinant porcine standards spanning a detection range of 15.6 to 1000 pg/mL, biotinylated detection reagent, streptavidin-HRP conjugate, chromogenic substrate, stop solution, and concentrated wash buffer. Standard protocol calls for 100 uL of sample per well with incubation and washing steps performed at room temperature. Reagents are shipped refrigerated and remain stable when stored cold until the expiration date; reconstituted standards should be aliquoted and frozen to limit freeze-thaw cycles.