Porcine KIAA0101/PCNA-associated factor ELISA Kit

From Assay Genie, this Porcine KIAA0101/PCNA-associated factor ELISA Kit is a high-quality tool for the accurate detection of KIAA0101/PCNA-associated factor levels in porcine samples. KIAA0101 interacts with PCNA (proliferating cell nuclear antigen) and plays a critical role in cell cycle regulation and DNA replication. Dysregulation of KIAA0101 has been linked to various diseases including cancer, making it a valuable biomarker for studying these conditions and developing potential treatments.

The kit is described as a sandwich ELISA with a competitive detection method coated with antigen and a colorimetric readout. The kit is suitable for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and should be stored sealed at 2-8°C without cryopreservation. Absorbance is read at 450 nm using a microplate reader.

Reported linearity in serum (n=10) is 87-103% at 1:2, 83-97% at 1:4, and 83-100% at 1:8; in EDTA plasma (n=10) 90-98%, 82-89%, and 81-100%; and in heparin plasma (n=10) 88-101%, 82-101%, and 84-100%. Recovery ranges are 92-104% (average 98) for serum, 85-104% (average 95) for EDTA plasma, and 86-101% (average 93) for heparin plasma.