Porcine Interleukin 8 (IL-8) ELISA Kit

CXC chemokines, particularly IL-8 (CXCL8), are central mediators of neutrophil-driven inflammation in swine and serve as informative biomarkers in studies of porcine respiratory disease complex, Actinobacillus pleuropneumoniae infection, PRRSV lung injury, and experimental sepsis models. The Assay Genie Porcine IL-8 ELISA Kit is a quantitative immunoassay that measures this chemokine in pig-derived specimens.

Configured as a 96-assay microplate format using matched antibody pairs and a colorimetric readout, the assay is validated across multiple porcine sample matrices, including serum samples, plasma samples, tissue homogenates, cell lysates, and cell culture supernatants. Typical research applications include tracking cytokine responses in experimental infection models, measuring IL-8 release from porcine alveolar macrophages, evaluating anti-inflammatory interventions in swine, and quantifying chemokine release from stimulated peripheral blood mononuclear cells.

The kit supports in vitro research on porcine immune function, vaccine adjuvant testing, and welfare-related inflammation biomarkers. Handling follows standard ELISA practice: unopened kits are refrigerated until first use, and reconstituted standards are aliquoted and stored frozen to limit freeze-thaw degradation. Each kit ships with a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, chromogen substrate, wash buffer, standard diluent, and a recombinant porcine IL-8 standard curve.