Porcine Interleukin 12A (IL-12A) ELISA Kit

A complete absorbance-based ELISA workflow from Assay Genie quantitatively measures the IL-12A subunit (p35, approximately 35 kDa) in porcine samples, supporting infectious-disease research, vaccine development, reproductive immunology, and immune-competence studies in swine. The kit targets the IL-12A alpha chain, which pairs with IL-12B (p40) to form the bioactive IL-12 p70 heterodimer, a cytokine central to Th1-type immune responses, NK cell activation, and IFN-gamma induction.

Validated exclusively for porcine reactivity, the assay accommodates individual serum samples, plasma samples, tissue homogenates, cell lysates, and cell culture supernatants across veterinary and pre-clinical specimen types. The plate-based protocol uses pre-coated wells, porcine-specific capture and detection antibodies, serial standard dilutions for curve generation, wash buffer, chromogenic substrate solution, and stop reagent, supplied together with running buffer components. Unopened reagents should be stored refrigerated until first use.

Researchers deploy this kit for monitoring inflammatory cytokine release following pathogen challenge (such as PRRSV, PCV2, or LPS stimulation), evaluating adjuvant efficacy in vaccine trials, profiling immune signatures in transplantation or wound-healing models, and benchmarking innate immune competence in breeding stock. The microplate layout supports both single-animal pilot studies and larger cohort screens across multiple experimental conditions.