Porcine Interferon Alpha (IFNa) ELISA Kit

The Porcine Interferon Alpha (IFNa) ELISA Kit from Assay Genie provides researchers with a quantitative tool for measuring IFN-α cytokine levels in swine-derived samples. Interferon-alpha is a key type I interferon involved in antiviral defense, innate immune signaling, and the regulation of adaptive immunity, making its accurate measurement essential in porcine infectious disease research, vaccine development studies, and investigations of respiratory or reproductive viral pathogens such as PRRSV and porcine circovirus.

Built on a plate-based immunoassay workflow, the kit pairs pre-coated capture antibodies with detection reagents to produce a measurable signal proportional to IFN-α concentration. Researchers can apply the kit to a spectrum of swine-derived specimens, including whole blood fractions, tissue homogenates prepared from lung, spleen, or lymph node, and cell culture supernatants from stimulated porcine peripheral blood mononuclear cells.

For optimal reagent stability, unopened kits should be stored refrigerated at 4°C; reconstituted standards and working detection antibody solutions require freezer storage at -20°C in aliquoted form to limit freeze-thaw cycles. The assay procedure typically requires approximately 100 µL of sample per well, with a total incubation-to-readout workflow that can be completed within a standard laboratory workday.

This Research Use Only product is supplied as a complete kit containing a pre-coated microplate, recombinant porcine IFN-α standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop reagent. Each lot is validated by Assay Genie to support reproducible quantification across the dynamic range of the assay, supporting immunology, virology, and swine biomedical research.