Porcine IL-17 ELISA Kit

Assay Genie supplies a sandwich ELISA kit intended for the quantitative measurement of interleukin-17 in porcine samples. The assay uses a double-antibody sandwich format with colorimetric detection and is suitable for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types.

Manufacturer-reported sensitivity is 9.375 pg/mL with a working range of 15.625-1000 pg/mL. The kit is stored at 2-8°C for 12 months. Reported recovery averages 96% for serum, 93% for EDTA plasma, and 93% for heparin plasma, with linearity values given for 1:2, 1:4 and 1:8 dilutions across those same sample types.

The procedure includes reagent equilibration, a primary incubation at 37°C for 90 minutes, biotin-labelled detection antibody binding at 37°C for 60 minutes, HRP-Streptavidin binding at 37°C for 30 minutes, TMB substrate development in the dark for 10-20 minutes, and absorbance measurement at 450 nm after addition of stop solution. The target analyte is catalogued under UniProt Q60I29 and is described by the manufacturer as a cytokine involved in immune responses and inflammation.