Porcine IGFBP4 ELISA Kit

From Assay Genie, this Porcine IGFBP4 ELISA Kit is a Sandwich ELISA, Double Antibody assay developed for the quantitative detection of Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) in porcine samples. Detection is colorimetric, with absorbance read at 450 nm after TMB substrate development, supporting research into growth factor regulation and its influence on cell growth, differentiation, and survival.

Reported performance includes a sensitivity of 0.188 ng/mL and an assay range of 0.313-20 ng/mL. Validated sample types are serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Linearity across 1:2, 1:4 and 1:8 dilutions was 95-105%, 85-103% and 86-104% in serum (n = 5); 88-96%, 88-100% and 83-101% in EDTA plasma (n = 5); and 81-100%, 80-100% and 80-98% in heparin plasma (n = 5). Spike-and-recovery averages were 94% for serum (range 89-102%), 95% for EDTA plasma (range 86-100%) and 96% for heparin plasma (range 92-100%).

The Uniprot reference for IGFBP4 is P24854, with listed synonyms IGFBP-4, IGFBP4, BP4, HT29-IGFBP and IBP4. Reagents are stored at 2-8°C for 12 months.