Porcine IFN gamma ELISA Kit

From Assay Genie, this Porcine IFN gamma ELISA Kit is a Sandwich ELISA, Double Antibody assay designed for the precise measurement of interferon gamma levels in porcine samples. Interferon gamma is a key cytokine involved in immune responses, playing a critical role in host defense against infections and in the regulation of inflammation, making it a valuable biomarker for studying immune function and developing potential treatments in porcine health and disease.

The assay has a sensitivity of 18.75 pg/mL and a standard range of 31.25-2000 pg/mL, using a colorimetric detection method with absorbance read at 450 nm following TMB substrate development. It is compatible with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with storage at 2-8°C for 12 months. Linearity across 1:2, 1:4 and 1:8 dilutions was 85-103%, 85-97% and 85-100% for serum (n = 5); 85-103%, 82-101% and 89-100% for EDTA plasma (n = 5); and 88-95%, 83-95% and 82-95% for heparin plasma (n = 5). Recovery ranges were 85-103% (average 95%) for serum (n = 5), 87-105% (average 96%) for EDTA plasma (n = 5), and 90-104% (average 96%) for heparin plasma (n = 5). Synonyms for the target include IFN-gamma, IFNG, IFG, IFI, and Type II Interferon (UniProt P17803).