Porcine High Mobility Group Protein 1 (HMG1) ELISA Kit

Quantify porcine HMGB1 in translational and veterinary research with this species-specific enzyme-linked immunosorbent assay from Assay Genie. HMGB1 (also called HMG1 or amphoterin) is a highly conserved nuclear DNA-binding protein that, when released from damaged or activated cells, acts as a damage-associated molecular pattern and drives late-phase inflammatory signalling. Because swine share close immunological and physiological similarity with humans, porcine HMGB1 measurement is frequently used to evaluate sepsis, systemic inflammation, traumatic injury, and post-surgical recovery in preclinical models.

The assay is calibrated for porcine samples and is supplied as a complete microplate kit containing all reagents required for 96 individual determinations. Researchers typically apply it to blood-derived fractions from swine, including clotted and anticoagulated preparations, as well as cell culture supernatants and tissue homogenates. Reagents should be kept refrigerated between uses and protected from light to preserve antibody binding activity. The kit is intended for research use only and is not validated for diagnostic applications.

Each plate is formatted in strip-wells, allowing researchers to run partial batches when sample volume is limited. Full standard curves are generated in duplicate to support accurate interpolation of unknown concentrations. For laboratories working across species, a parallel panel of HMGB1 kits may be used to compare inflammatory kinetics between porcine and other mammalian models.