Porcine Hepatocyte Growth Factor (HGF) ELISA Kit

This porcine-specific immunoassay from Assay Genie is configured for quantitative measurement of Hepatocyte Growth Factor (HGF) across porcine serum samples, plasma-derived fractions, and tissue homogenate matrices. The kit supports 96 assays per plate and produces an absorbance-based readout compatible with standard microplate readers, making it straightforward to integrate into existing ELISA workflows focused on swine cytokine and growth factor profiling.

HGF is a pleiotropic signaling molecule best known for activating the c-MET receptor tyrosine kinase, driving hepatocyte proliferation, epithelial-mesenchymal transition, and tissue regeneration processes. In swine models, which are frequently used in translational hepatology, cardiovascular, and transplant research, circulating HGF concentrations serve as a biomarker of hepatic injury, fibrotic remodeling, and acute-phase responses. Researchers measuring this analyte commonly correlate serum HGF titers with histopathology, ALT/AST release, or regenerative outcomes following partial hepatectomy or toxicant exposure.

Each kit is supplied as a complete set of pre-coated microwell strips, detection antibody, standard diluent, wash buffer, substrate solution, and stop reagent. The 96 assays format accommodates moderate-throughput studies and supports batch analysis of experimental cohorts. Recommended handling includes equilibrating reagents to room temperature prior to use and aliquoting standards to limit freeze-thaw cycles that could degrade the recombinant HGF calibrator. Intended for research use only.

This kit is validated specifically for swine specimens, supporting species-appropriate quantification without the cross-reactivity concerns that arise when applying human or rodent HGF assays to pig samples.